二乙烯亚胺对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果Inactivation Effect of Binary Ethylenimine on Type Ⅱ Poliovirus Sabin Strain
王白燕;张新文;蔡玮;马磊;高菁霞;孙明波;姜述德;李卫东;杨静思;廖国阳;
摘要(Abstract)：
目的观察二乙烯亚胺(Binary ethylenimine,BEI)对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果。方法在不同温度(37、33、28和25℃)下采用4种浓度(0.002、0.001、0.0005和0.00025mol/L)的BEI及0.0925mg/ml的甲醛对脊髓灰质炎病毒Ⅱ型Sabin株进行灭活,采用微量滴定法测定病毒感染性滴度,ELISA法检测灭活病毒液D抗原含量,并进行病毒灭活验证试验。结果在37℃条件下,0.002mol/L的BEI能在48h内完全灭活病毒,灭活后的病毒液D抗原含量回收率为90.6%,显著高于甲醛灭活的回收率(60.6%)。经验证,灭活彻底,无活病毒存在。结论 BEI对脊髓灰质炎病毒Ⅱ型Sabin株的D抗原破坏小,能较好地保持疫苗的有效成分。
关键词(KeyWords)： 脊髓灰质炎病毒;二乙烯亚胺;病毒灭活
基金项目(Foundation): 云南省重点新产品开发项目(2008CA035);; 国家重大新药创制(2008ZX09101-031);; 云南省技术创新人才项目(2003PY07);; 国家支撑计划(2008BAI66B04)
作者(Authors): 王白燕;张新文;蔡玮;马磊;高菁霞;孙明波;姜述德;李卫东;杨静思;廖国阳;
DOI: 10.13200/j.cjb.2010.06.94.wangby.020
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