周静;王轶楠;柳忠辉;冯野;陈芳芳;台桂香;

• 1:吉林大学白求恩医学院免疫学教研室
• 2:吉林大学中日联谊医院外科

摘要(Abstract)：

目的探讨脂多糖(LPS)诱导小鼠巨噬细胞系RAW264.7细胞活化凋亡的作用。方法体外培养小鼠巨噬细胞系RAW264.7细胞,分别用0.5、1.0、2.5μg/mlLPS刺激RAW264.7细胞24h,一氧化氮(NO)试剂盒检测细胞培养上清中NO水平;用1.0μg/mlLPS分别刺激细胞3d和6d,台盼蓝拒染法检测细胞增殖情况;用1.0μg/mlLPS刺激细胞6d,流式细胞术分析细胞周期及细胞凋亡情况。结果经LPS刺激后24h,RAW264.7细胞培养上清中NO含量明显增加,且具有剂量依赖性;LPS刺激3d和6d后,细胞的增殖均受到抑制,且呈时间依赖性;LPS刺激6d时,细胞周期被阻滞在S期,并出现明显的凋亡。结论LPS具有诱导小鼠巨噬细胞系RAW264.7细胞活化凋亡的作用。

关键词(KeyWords)： 脂多糖;巨噬细胞;活化凋亡

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30571688,30671953,30801005)

作者(Authors): 周静;王轶楠;柳忠辉;冯野;陈芳芳;台桂香;

DOI: 10.13200/j.cjb.2009.02.37.zhouj.016

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