硫酸软骨素B佐剂对甲型肝炎病毒疫苗诱导小鼠体液免疫应答的影响Effect of chondroitin sulfate B adjuvant on humoral immune response induced by hepatitis A virus vaccine in mice
王陈芸;孙静;李萍;余柄廷;李建芳;李彦涵;王海漩;乌美妮;胡凝珠;胡云章;
摘要(Abstract)：
目的探讨硫酸软骨素B(chondroitin sulfate B,CSB)佐剂对甲型肝炎病毒((hepatitis A virus,HAV)疫苗诱导小鼠体液免疫应答的影响,以评价其佐剂效果。方法将ICR小鼠随机分成9组:甲型肝炎减毒活疫苗Hep A-l18 EU+不同剂量CSB(5、25、45、65、85、105μg)组、阴性对照组(生理盐水)、抗原对照组(Hep A-l 18 EU)和铝佐剂对照组[Hep A-l 18 EU+Al(OH)3300μg],每组8只,各组均经皮下注射ICR小鼠,200μl/只。于免疫后的第4、8、12和16周,采用间接ELISA法检测小鼠血清中抗-HAV特异性Ig G抗体水平。在实验期间,观察小鼠的健康状况,并于免疫16周后,取CSB 65μg组及阴性对照组小鼠肾脏、脾脏、肝脏、肺脏、心脏各主要器官,制备病理切片,进行对比观察。结果各组小鼠免疫后第4、8、12、16周,均产生抗-HAV Ig G抗体,除阴性对照组外,抗体水平均随时间的延长逐渐升高,于第8周达峰值,此后逐渐下降。CSB 65μg组抗体可长时间维持在高水平,至免疫后第12、16周,均高于抗原对照组(P<0.05),略高于铝佐剂对照组,最佳浓度为65μg/只。实验期间,各组小鼠均未出现异常;CSB65μg组小鼠肾脏、脾脏、肝脏、肺脏、心脏组织均未观察到病变。结论 CSB可明显增强HAV抗原诱导小鼠的体液免疫应答,具有成为新型疫苗佐剂的研发价值。
关键词(KeyWords)： 硫酸软骨素B;佐剂;甲型肝炎病毒疫苗;体液免疫
基金项目(Foundation): 2013年云南省高技术产业发展项目计划:基因工程药物关键技术研究及新产品开发应用;云南省社会发展新药专项“治疗2型糖尿病Ⅰ类新药生物制品RBP4临床前研究”(2013BC011)
作者(Authors): 王陈芸;孙静;李萍;余柄廷;李建芳;李彦涵;王海漩;乌美妮;胡凝珠;胡云章;
DOI: 10.13200/j.cnki.cjb.000843
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