结肠癌源性exosomes的分离及其相关免疫学性质Isolation and immunologic characteristics of exosomes derived from colon carcinoma cells
冯业童;刘朋飞;吴昊昱;刘迪;董超;吴璇;周余来;孙波;
摘要(Abstract)：
目的分离结肠癌细胞株的exosomes,并分析其在致敏抗原呈递细胞及激活相关效应细胞过程中的作用。方法差速离心法分离体外培养的正常exosomes和经热休克处理的sw1116细胞(Heat shocked sw1116,HS-sw1116)分泌的exosomes(Heat shocked exosomes,HS-Exo),并在电子显微镜下观察exosomes和HS-Exo的形态结构;SDS-PAGE初步分析exosomes和HS-Exo的蛋白组分,CCK-8法检测其促外周血单个核细胞(Peripheral blood monouclear cells,PBMCs)增殖的能力。结果电子显微镜观察,exosomes和HS-Exo的形态学结构无明显差异,其平均直径约为150 nm;exosomes和HS-Exo的蛋白条带分布情况基本相同,在高相对分子质量区域蛋白分布较多;exosomes比sw1116细胞更易引起PBMCs的增殖反应,HS-sw1116细胞和HS-Exo促PBMCs增殖的作用比sw1116细胞和exosomes更明显(P<0.05)。结论结肠癌sw1116细胞株可分泌exosomes,其比肿瘤细胞更易引起PBMCs的增殖,热休克处理可进一步增强细胞和exosomes的促PBMCs增殖的能力,exosomes在结肠癌免疫治疗方面具有重要的应用价值。
关键词(KeyWords)： 结肠癌;exosomes;热休克;免疫治疗
基金项目(Foundation): 吉林省卫生厅科研课题(2009Z043)
作者(Authors): 冯业童;刘朋飞;吴昊昱;刘迪;董超;吴璇;周余来;孙波;
DOI: 10.13200/j.cjb.2012.12.76.fengyt.036
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