连海峰;吴小翎;刘倩;

• 1:重庆医科大学附属第二医院消化内科
• 2:滨州医学院附属医院消化内科

摘要(Abstract)：

目的探讨神经纤毛蛋白2(Neuropilin2,NRP2)干扰质粒对胃腺癌SGC7901细胞株生长、侵袭及转移的影响。方法构建NRP2特异的小RNA干扰质粒shRNA-NRP2,采用脂质体介导的方法将质粒转染入人胃腺癌SGC7901细胞株,Western blot法检测NRP2蛋白的表达;Transwell试验检测胃癌细胞体外迁移和侵袭能力的变化;MTT试验检测胃癌细胞的增殖水平。结果 shRNA-NRP2转染SGC7901细胞后,NRP2蛋白表达明显下降,细胞的侵袭、迁移能力明显受抑制,而细胞的增殖水平无显著变化。结论 shRNA-NRP2干扰质粒可下调胃腺癌SGC7901细胞株NRP2蛋白的表达,进而抑制细胞的侵袭、转移能力,提示NRP2可能成为胃癌分子治疗的靶标。

关键词(KeyWords)： 神经纤毛蛋白质2;胃肿瘤;RNA干扰

Abstract:

Keywords:

基金项目(Foundation): 重庆市卫生局医学科研项目(2008-1-26)

作者(Authors): 连海峰;吴小翎;刘倩;

DOI: 10.13200/j.cjb.2010.10.32.lianhf.032

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