王贝贝;邓守全;马德慧;王学理;

• 1:内蒙古民族大学动物科技学院

摘要(Abstract)：

近年来,我国许多鸡、鸭主要养殖区相继出现以产蛋率急剧下降,卵巢出血为主要病变,以死淘率高、发病急、传播迅速为特点的禽类疾病。目前认为该病由一种新型的未分类黄病毒——坦布苏病毒引起,给中国的禽类养殖业造成了巨额损失。本文就禽坦布苏病毒病的生物学特性、临床症状及病理变化、基因组及编码蛋白、检测方法的建立等研究进展作一综述,以期为该病的深入研究提供参考。

关键词(KeyWords)： 禽坦布苏病毒;生物学特性;检测方法

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划(2016YFD0501005);; 内蒙古自然科学基金(2013MS0404)

作者(Authors): 王贝贝;邓守全;马德慧;王学理;

DOI: 10.13200/j.cnki.cjb.002061

参考文献(References)：

• [1]WU L,LIU J X,CHEN P C,et al.The sequential tissue distribution of duck Tembusu virus in adult ducks[J].Biomed Res Int,2014,2014:703930.doi:10.1155/2014/703930.
• [2]THONTIRAVONG A,NINVILAI P,TUNTERAK W,et al.Tembusu-related flavivirus in ducks,Thailand[J].Emerg Infect Dis,2015,21(12):2164-2167.
• [3]李玉峰.鸭黄病毒感染研究进展[J].中国家禽,2011,33(17):30-32.
• [4]ZOU Z,LIU Z G,JIN M L.Efficient strategy to generate a vectored duck enteritis virus delivering envelope of duck Tembusu virus[J].Viruses,2014,6(6):2428-2443.
• [5]LI L L,AN H J,SUN M H,et al.Identification and genomic analysis of two duck-origin Tembusu virus strains in southern China[J].Virus Genes,2012,45(1):105-112.
• [6]LI X S,SHI Y,LIU Q F,et al.Airborne transmission of a novel Tembusu virus in ducks[J].J Clin Microbiol,2015,53(8):2734-2736.
• [7]LI S,LI X X,ZHANG L J,et al.Duck Tembusu virus exhibits neurovirulence in BALB/c mice[J].Virol J,2013,10:260.
• [8]SUN L,LI Y X,ZHANG Y,et al.Adaptation and attenuation of duck Tembusu virus strain Du/CH/LSD/110128 following serial passage in chicken embryos[J].Clin Vaccine Immunol,2014,21(8):1046-1053.
• [9]YIN X C,LV R,CHEN X D,et al.Detection of specific antibodies against Tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay[J].J Clin Microbiol,2013,51(7):2400-2402.
• [10]SUN M H,HU Q L,DONG J W,et al.Isolation,identification and E gene phylogenetic analysis of a duck-origin Tembusu virus from Jiangmen[J].Guangdong Agr Sci,2012,39(11):158-160.(in Chinese)孙敏华,胡奇林,董嘉文,等.鸭坦布苏病毒江门株的分离鉴定及其E基因系统进化分析[J].广东农业科学,2012,39(11):158-160.
• [11]YUAN S,LI J P,WANG M R,et al.Isolation and preliminary identification of a novel flavivirus strain from geese[J].China Anim Husb Vet Med,2012,39(9):47-50.(in Chinese)袁生,李金平,王敏儒,等.鹅源黄病毒的分离和初步鉴定[J].中国畜牧兽医,2012,39(9):47-50.
• [12]TENG Q Y,YAN P X,ZHANG X,et al.A novel flavivirus causing duck egg drops and death[J].Chin J Anim Infect Dis,2010,18(6):1-4.(in Chinese)滕巧泱,颜丕熙,张旭,等.一种新的黄病毒导致蛋鸭产蛋下降及死亡[J].中国动物传染病学报,2010,18(6):1-4.
• [13]LIAO M,MU X D,GENG Y,et al.The primary study on virus isolation of duck infectious egg failings[J].Chin J Anim Infect Dis,2011,19(1):22-26.(in Chinese)廖敏,牟小东,耿阳,等.鸭传染性产蛋减少症(暂定名)的病原分离初报[J].中国动物传染病学报,2011,19(1):22-26.
• [14]GUO J J,YAN Q L,SUN X H.Overview of the non-classified faviviruses[J].J Inspct Quarant,2012,22(6):48-51,22.(in Chinese)郭金金,燕清丽,孙肖红.未分类黄病毒研究进展[J].检验检疫学刊,2012,22(6):48-51,22.
• [15]HAN K K,ZHAO D M,LIU Y Z,et al.Quantitative proteomic analysis of duck ovarian follicles infected with duck Tembusu virus by label-free LC-MS[J].Front Microbiol,2016,7:463.
• [16]LIU Z G,SUN Q S,YAO R,et al.Research progress on duck tembusu virus[J].Chin J Anim Infect Dis,2013,21(1):81-86.(in Chinese)刘志刚,孙青松,姚蓉,等.鸭坦布苏病毒研究进展[J].中国动物传染病学报,2013,21(1):81-86.
• [17]MA X L,YU K X,GAO F,et al.Biological characteristics of BZ isolate of duck flavivirus[J].China Poul,2011,33(21):12-14.(in Chinese)马秀丽,于可响,高凤,等.鸭黄病毒BZ株的生物学特性研究[J].中国家禽,2011,33(21):12-14.
• [18]TANG Y,DIAO Y,GAO X,et al.Analysis of the complete genome of Tembusu virus,a flavivirus isolated from ducks in China[J].Transbound Emerg Dis,2012,59(4):336-343.
• [19]MUKHOPADHYAY S,KUHN R J,ROSSMANN M G.A structural perspective of the flavivirus life cycle[J].Nat Rev Microbiol,2005,3(1):13-22.
• [20]YAN P,ZHAO Y,ZHANG X,et al.An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China[J].Virology,2011,417(1):1-8.
• [21]YU K,SHENG Z Z,HUANG B,et al.Structural,antigenic and evolutionary characterizations of the envelope protein of newly emerging duck Tembusu virus[J].PLo S One,2013,8(8):e71319.doi:10.1371/journal.pone.0071319.
• [22]LINDENBACH B D,RICE C M.Molecular biology of flaviviruses[J].Adv Virus Res,2003,59(1):23-61.
• [23]ZHENG J,ZHAO Q Z,ZHAO Y,et al.The structure and the function of NS2-3/NS3 protein of pestivirus[J].Chin J Virol,2007,23(3):235-239.(in Chinese)郑杰,赵启祖,赵耘,等.黄病毒NS2-3/NS3蛋白的结构与功能[J].病毒学报,2007,23(3):235-239.
• [24]SAIYASOMBAT R,DORMAN K S,GARCIA-REJON J E,et al.Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico[J].Arch Virol,2010,155(6):983-986.
• [25]HOSHINO K,ISAWA H,TSUDA Y,et al.Genetic characterization of a new insect flavivirus isolated from Culex pipiens mosquito in Japan[J].Virology,2007,359(2):405-414.
• [26]LIU M,LIU C,LI G,et al.Complete genomic sequence of duck flavivirus from China[J].J Virol,2012,86(6):3398-3399.
• [27]HALSTEAD S B,THOMAS S J.Japanese encephalitis:new options for active immunization[J].Clin Infect Dis,2010,50(8):1155-1564.
• [28]WANG L C,LI Q Y,CHEN R A,et al.Duck hemorrhagic ovaritis in Guangdong province and the construction of a RTPCR detection method[J].Guangdong J Anim Vet Sci,2011,36(6):27-28,31.(in Chinese)王林川,李庆阳,陈瑞爱,等.鸭出血性卵巢炎广东病例报道及其RT-PCR方法的建立[J].广东畜牧兽医科技,2011,36(6):27-28,31.
• [29]LI G P.Development and application of RT-PCR method for detection of duck tembusu virus[J].China Anim Husb Vet Med,2013,40(1):69-72.(in Chinese)李国平.鸭坦布苏病毒RT-PCR诊断方法的建立与应用[J].中国畜牧兽医,2013,40(1):69-72.
• [30]赵虹,谢芝勋,谢丽基,等.鸭坦布苏病毒和禽流感病毒多重PCR检测方法的建立[J].畜牧与兽医,2015,47(3):90-93.
• [31]SUN M H,LI L L,DONG J W,et al.Duplex PCR assay for the detection of duck tembusu virus and egg drop syndrome virus[J].Guangdong J Anim Vet Sci,2015,40(1):29-33.(in Chinese)孙敏华,李林林,董嘉文,等.鸭坦布苏病毒和产蛋下降综合征病毒二重PCR检测方法的建立[J].广东畜牧兽医科技,2015,40(1):29-33.
• [32]ZHANG Y F,XIE Z X,XIE L J,et al.Development of duplex real-time RT-PCR assay for detection of duck tembusu virus and duck plague virus[J].China Anim Husb Vet Med,2014,41(3):77-82.(in Chinese)张艳芳,谢芝勋,谢丽基,等.鸭坦布苏病毒和鸭瘟病毒二重荧光定量RT-PCR方法的建立[J].中国畜牧兽医,2014,41(3):77-82.
• [33]ZHANG Y F,XIE Z X,XIE L J,et al.Establishment of a duplex RT-PCR assay for detection of duck Tembusu virus and duck circovirus[J].Heilongjiang Anim Sci Vet Med,2014,57(12):44-47.(in Chinese)张艳芳,谢芝勋,谢丽基,等.鸭坦布苏病毒与鸭圆环病毒二重RT-PCR方法的建立[J].黑龙江畜牧兽医,2014,(12):44-47.
• [34]ZENG T T,XIE Z X,XIE L J,et al.Development of duplex real-time fluorescence RT-PCR assay for differential detection of tembusu virus and egg drop syndrome virus[J].China Poul,2015,37(1):17-21.(in Chinese)曾婷婷,谢芝勋,谢丽基,等.应用二重实时荧光定量RTPCR鉴别坦布苏病毒与产蛋下降综合征病毒[J].中国家禽,2015,37(1):17-21.
• [35]TI J F,LI Z J,DOU Y G,et al.Development of real-time PCR assay to detect tembusu virus in mice or nurine cells[J].Chin J Vet Sci,2016,36(6):917-922.(in Chinese)提金凤,李志杰,窦砚国,等.检测鼠或鼠源细胞中坦布苏病毒荧光定量PCR方法的建立[J].中国兽医学报,2016,36(6):917-922.
• [36]ZHANG R R,WEN G Y,LUO Q P,et al.Establishment of fluorescence quantitative RT-PCR assay for detecting duck flavivirus[J].Hubei Agr Sci,2013,52(23):5811-5813.(in Chinese)张蓉蓉,温国元,罗青平,等.鸭黄病毒荧光定量PCR检测方法的建立[J].湖北农业科学,2013,52(23):5811-5813.
• [37]WAN C H,ZHU H X,SHI S H,et al.Establishment of a SYBR GreenⅠ-based real-time RT-PCR for rapid detection of duck tembusu virus[J].Chin J Vet Sci,2013,33(7):973-978.(in Chinese)万春和,朱海侠,施少华,等.鸭坦布苏病毒SYBR GreenⅠ实时荧光定量检测方法的建立[J].中国兽医学报,2013,33(7):973-978.
• [38]YU C M,DIAO Y X,TANG Y,et al.Fluorescence quantitative RT-PCR assay for detection of tembusu virus[J].Sci Agr Sin,2012,45(21):4492-4500.(in Chinese)于春梅,刁有祥,唐熠,等.坦布苏病毒荧光定量RT-PCR方法的建立[J].中国农业科学,2012,45(21):4492-4500.
• [39]HUANG X M,ZHAO D M,LIU Y Z,et al.Establishment and application of a nested RT-PCT method for detection of avian flavivirus[J].Anim Husb Vet Med,2012,44(6):1-4.(in Chinese)黄欣梅,赵冬敏,刘宇卓,等.禽黄病毒套式RT-PCR检测方法的建立及应用[J].畜牧与兽医,2012,44(6):1-4.
• [40]YAN P X,LI X G,WU X G,et al.Rapid identification of duck tembusu virus by the nested RT-PCR[J].Chin J Anim Infect Dis,2011,19(3):34-37.(in Chinese)颜丕熙,李国新,吴晓刚,等.应用套式RT-PCR快速检测鸭坦布苏病毒[J].中国动物传染病学报,2011,19(3):34-37.
• [41]TANG Y,DIAO Y X,GAO X H,et al.Development of a semi-nested RT-PCR assay for detection of duck flavivirus[J].Chin J Vet Sci,2012,32(4):517-520.(in Chinese)唐熠,刁有祥,高绪慧,等.鸭黄病毒感染逆转录半套式PCR检测方法的建立[J].中国兽医学报,2012,32(4):517-520.
• [42]YUAN P,WANG B,XU C T,et al.Establishment and application of quantitatively competitive PCR system for DTMUV detection[J].Shandong Agr Sci,2015,47(3):113-117.(in Chinese)袁朋,王斌,许传田,等.鸭坦布苏病毒竞争定量PCR检测方法的建立与应用[J].山东农业科学,2015,47(3):113-117.
• [43]ZHANG W,LU M Y,HUANG Q H,et al.Application and comparison of fluorescent reagents in detection of duck tembusu virus by loop-mediated isothermal amplification[J].Chin Vet Sci,2014,44(4):406-411.(in Chinese)张伟,逯茂洋,黄庆华,等.荧光显色技术在鸭坦布苏病毒LAMP检测方法中的应用及比较[J].中国兽医科学,2014,44(4):406-411.
• [44]GAO X H,DIAO Y X,TANG Y,et al.Preparation of digoxigenin-labeled DNA probe for detection of duck flavivirus and its application[J].Chin J Vet Sci,2012,32(4):525-528.(in Chinese)高绪慧,刁有祥,唐熠,等.探针检测鸭黄病毒的地高辛标记DNA的制备与应用[J].中国兽医学报,2012,32(4):525-528.
• [45]YUN T,NI Z,HUA J,et al.Development of a one-step real time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus[J].J Virol Methonds,2012,181(2):148-154.
• [46]ZHENG X L,ZHAO M,WANG Q,et al.Establishment of chemiluminescence immunoassay for detection of duck flavivirus antibody[J].Chin Vet Sci,2015,45(9):908-911.(in Chinese)郑小龙,赵明,王群,等.鸭黄病毒抗体的化学发光免疫检测方法的建立[J].中国兽医科学,2015,45(9):908-911.
• [47]ZHU Y C,SUN J G,LIN M,et al.Development of a rapid detection of duck tembusu virus using colloid gold immunochromatography assay[J].J Agr Univ of Hebei,2014,37(5):100-103.(in Chinese)朱玉婵,孙继国,林密,等.胶体金免疫层析试纸条快速检测鸭坦布苏病毒方法的建立[J].河北农业大学学报,2014,37(5):100-103.
• [48]LI X,LI G,TENG Q,et al.Development of a blocking ELISA for detection of serum neutralizing antibodies against newly emerged duck Tembusu virus[J].PLo S One,2012,7(12):e53026.doi:10.1371/journal.pone.0053026.
• [49]JI X W,YAN L P,YAN P X,et al.Establishment of an indirect ELISA for detection of antibody against duck tembusu virus[J].Chin J Prev Vet Med,2011,33(8):630-634.(in Chinese)姬希文,闫丽萍,颜丕熙,等.鸭坦布苏病毒抗体间接ELISA检测方法的建立[J].中国预防兽医学报,2011,33(8):630-634.
• [50]TI J F,LI Z J,LI X L,et al.Expression of NS1 protein of tembusu virus and development of indirect ELISA assay[J].Acta Vet Zoot Sin,2016,47(5):970-977.(in Chinese)提金凤,李志杰,李秀丽,等.坦布苏病毒NS1蛋白的表达及ELISA检测方法的建立[J].畜牧兽医学报,2016,47(5):970-977.