李鹏;马艳娇;李建华;宫鹏涛;杨举;李赫;欧阳红生;张西臣;

• 1:黑龙江八一农垦大学动物科技学院
• 2:吉林大学畜牧兽医学院

摘要(Abstract)：

目的探讨COL1A1基因干扰对人结肠癌HCT-8细胞增殖的影响。方法将COL1A1基因干扰质粒pGPU6/GFP/Neo-COL1A1(RNAi COL1A1)和阴性对照质粒pGPU6/GFP/Neo-shNC(PGNsN)瞬时转染入HCT-8细胞,并设空白对照组。转染后48～72 h,采用半定量RT-PCR法检测COL1A1基因在细胞中的转录水平;MTT法检测细胞的增殖活力;并将各组细胞接种至BALB/c小鼠上皮组织中,每隔6 d分别测量小鼠体内肿瘤大小。结果 RNAi COL1A1组HCT-8细胞COL1A1基因的mRNA转录水平较PGNsN组和空白对照组显著降低(P<0.01)。RNAi COL1A1组与PGNsN组和空白对照组比较,细胞的增殖活力有所降低,第4～7天差异有统计学意义(P<0.05),其中在第5和第6天差异极显著(P<0.01)。RNAi COL1A1组小鼠体内肿瘤大小与PGNsN组和空白对照组比较,前18 d差异无统计学意义(P>0.05);在第24天,显著小于两个对照组(P<0.05);在第30天,差异极显著(P<0.01)。结论 COL1A1基因被干扰后,能够抑制HCT-8细胞增殖,为COL1A1成为癌症治疗的候选基因提供了实验依据。

关键词(KeyWords)： COL1A1基因;RNA干扰;结肠癌;细胞增殖

Abstract:

Keywords:

基金项目(Foundation): 国家重大基础研究计划资助项目(2006CB910505)

作者(Authors): 李鹏;马艳娇;李建华;宫鹏涛;杨举;李赫;欧阳红生;张西臣;

DOI: 10.13200/j.cjb.2012.04.35.lip.032

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