鸡痘病毒在鸽体内的分布及毒性Distribution and Toxicity of Fowlpox Virus in Pigeon
金明兰;鲁会军;马鸣潇;郑敏;刘慧娟;金宁一;
摘要(Abstract):
目的检测鸡痘病毒(Fowlpox virus,FPV)在鸽体内的分布及毒性。方法将鸡痘病毒经翅膀内侧无血管处刺种鸽子,50μl/只,对照组经相同部位刺种等量生理盐水。刺种后逐日进行临床观察,分别于刺种后6h、12h、1d、3d、7d、10d、15d和21d,剖杀鸽子,进行内脏器官的病理组织学检测,采用PCR法检测FPV在鸽体内的分布。结果在试验期间,幼鸽精神状态良好,采食、饮水正常,刺种后15d,临床症状基本消失;各组织未见明显的病理变化;刺种后6h,在刺种肌肉内检测到病毒DNA;刺种后3d和7d,在所有组织内均检测到病毒DNA;刺种后10d,在肌肉和脾脏内可检测到病毒DNA;刺种后15d,所有组织内均未检测到病毒DNA;对照组未检测到病毒DNA。结论鸡痘病毒在鸽体内存留时间短,无毒性,生物安全性良好。
关键词(KeyWords): 鸡痘病毒;鸽;病毒分布;毒性
基金项目(Foundation): “863”重大项目(2006AA10A205);; 科技支撑项目(2006BAD06A05)
作者(Authors): 金明兰;鲁会军;马鸣潇;郑敏;刘慧娟;金宁一;
DOI: 10.13200/j.cjb.2010.10.29.jinml.005
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